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The enzyme TPH, which is accountable for the limiting step in the production of serotonin by vitamin D, is markedly induced by the metabolically active vitamin D metabolite 1,25(OH)2D in serotonergic neuron [10, 11]

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The voltage for flattening protein was set at 80 V, while for the separation step, the voltage was increased to 100 V, and the transfer step used 0.4 A for 90 min